Table 1

Number of cells (× 106) of leucocyte subsets in peripheral blood, lung parenchyma and bronchoalveolar lavage fluid (BALf).

BLOOD

Leukocyte subsets (× 106) in blood, lungs and BAL fluid post OVA treatment.


Total

WBCs

Lymphocytes

Monocytes

Neutrophils

Eosinophils

Basophils


CD3+

B220+


WA

11.9

± 2.4

5.54

± 1.17

3.08

± 1.05

0.38

± 0.185

3.648

± 0.184

0.223

± 0.014

0.066

± 0.001


WO

24.72

± 6.41

7.31

± 1.31

5.93

± 1.17

1.62

± 0.35

7.97

± 1.14

0.74

± 0.36

1.05

± 0.02


NOXA

14.2

± 3.012

5.7

± 1.865

2.99

± 1.033

0.77

± 0.053

4.56

± 1.074

0.133

± 0.043

0.028

± 0.002


NOXO

25.625

± 4.063

6.186

± 1.076

6.742

± 0.964

2.424

± 1.076

8.741

± 0.064

1.061 *

± 0.004

0.556

± 0.003


DKOA

11.7

± 2.76

4.69

± 1.06

2.467

± 0.96

0.63

± 0.12

3.74

± 1.27

0.106

± 0.07

0.02

± 0.001


DKOO

27.56

± 2.79

6.61

± 1.49

7.27

± 2.01

2.6

± 0.47

9.39

± 2.44

1.14 *

± 0.46

0.59

± 0.14


Lungs


Total

Lymphocytes

Monocytes

Neutrophils

Eosinophils

Basophils/

Mϕ


WBCs

CD3+

B220+

Mast cells


WA

1.86

± 0.543

0.179

± 0.021

0.035

± 0.002

0.826

± 0.054

0.319

± 0.054

0

0

0.497

± 0.015


WO

12.35

± 3.96

4.87

± 1.05

0.09

± 0.01

0.37

± 0.09

1.47

± 0.43

2.22

± 0.76

0.046

± 0.012

3.09

± 1.08


NOXA

5.49 #

± 1.064

0.446 #

± 0.002

0.113 #

± 0.001

2.593 #

± 0.76

0.892 #

± 0.011

0

0

1.489 #

± 0.016


NOXO

22.6 *

± 7.544

4.084

± 1.075

1.29 *

± 0.643

3.171 *

± 1.075

4.337 *

± 1.074

4.102 *

± 1.066

0.089 *

± 0.002

5.682

± 0.064


DKOA

3.78 #

± 1.07

0.3

± 0.14

0.07

± 0.01

1.78 #

± 0.54

0.61

± 0.02

0.001

± 0.0001

0

1.02 #

± 0.29


DKOO

21.66 *

± 408

3.89

± 1.06

0.43 *

± 0.16

3.03 *

± 0.89

4.16 *

± 1.33

3.93 *

± 1.13

0.08 *

± 0.002

5.44 *

± 1.26


BAL fluid (2 lungs)


WA

0.8

± 0.013

0

0

0.013

± 0.004

0

0

0

0.787

± 0.011


WO

9.46

± 0.89

1.64

± 0.21

1.54

± 0.021

1.26

± 0.03

0.86

± 0.01

4.26

± 0.07

0.04

± 0.002

0.56

± 0.004


NOXA

0.63

± 0.018

0

0

0.014

± 0.002

0

0

0

0.616

± 0.003


NOXO

16 *

± 3.159

2.04

± 0.754

1.213

± 0.003

2.08

± 0.074

2.427 *

± 1.066

6.741 *

± 2.076

0.051

± 0.001

1.568 *

± 0.72


DKOA

0.865 ± 0.01

0

0

0.025 ± 0.002

0

0

0

0.84 ± 0.18


DKOO

15.89 *

± 4.03

2 ± 0.86

1.22 ± 0.66

2.05 ± 0.75

2.415 *± 1.01

6.67 *

± 1.14

0.05

± 0.003

1.55 *

± 0.63


Number of cells (× 106) of leucocyte subsets in peripheral blood, lung parenchyma and bronchoalveolar lavage fluid (BALf).


T cells

B cells

Monocytes

Neutrophils

Eosinophils

Basophils


Lungs

WO

0.6

0.015

0.22

0.18

3

0.04


NOXO

0.6

0.19*

1.3

0.49*

3.8

0.16*


DKOO

0.58

0.05

1.1

0.44*

3.4

0.13*


BALf

WO

0.22

0.25

0.77

0.107

5.75

0.03


NOXO

0.32

0.17

0.85

0.27*

6.3

0.09


DKOO

0.51

0.16

0.78

0.26*

5.8

0.08


Total white blood cells were counted by a Beckman Coulter particle counter and subsets quantified using specific antibody staining in FACS and corroborated by morphological assessment by light microscopy and also by hemavet using the specifics of mouse. The table shows data averaged from three independent experiments ± SEM. * denotes p value < 0.05 compared to values from post-OVA wildtype mice and # denotes p value < 0.05 compared to alum treated wildtype. Abbreviations used are: WA = wildtype +alum, WO = wildtype + OVA, NOXA = gp91phox-/- + alum, NOXO = gp91phox-/- + OVA, DKOA = MMP12-gp91phox double knockout + alum, DKOO = MMP12-gp91phox double knockout + OVA. (n = 5/group). Recruitment index: Inflammatory hematopoietic cells found in lungs and BALf expressed as a fraction of corresponding cells in circulation. Number of cells (× 106) in lungs and BALf were the numerator and the number of cells (× 106) in circulating blood was the denominator in the fraction calculated to show recruitment index or inflammatory cells recruited from circulation to lung parenchyma and airways in response to the OVA allergic challenge. * denotes statistically significant change (p value < 0.01)

Banerjee and Henderson Clinical and Molecular Allergy 2012 10:2   doi:10.1186/1476-7961-10-2

Open Data