Research
Recombinant phospholipase A1 (Ves v 1) from yellow jacket venom for improved diagnosis of hymenoptera venom hypersensitivity
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* Corresponding authors: Markus Ollert ollert@lrz.tu-muenchen.de - Edzard Spillner spillner@chemie.uni-hamburg.de
1 Institute of Biochemistry and Molecular Biology, Department of Chemistry, University of Hamburg, Germany
2 PLS-Design GmbH, Hamburg, Germany
3 Clinical Research Division of Molecular and Clinical Allergotoxicology, Department of Dermatology and Allergy, Technische Universität München, Germany
Clinical and Molecular Allergy 2010, 8:7 doi:10.1186/1476-7961-8-7
Published: 1 April 2010Additional files
Additional file 1:
DLS measurement of rVes v 5. Dynamic light scattering measurements were carried out using the Spectroscatterer 201 (RiNA GmbH). Protein concentration of rVes v 5 was 0.12 mg/ml in 50 mM sodium phosphate, pH 7.6. rVes v 5 exhibited clear monodispersity with a hydrodynamic radius of 2.6 +/- 0.41 nm.
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Additional file 2:
Circular dichroism spectroscopy of rVes v 5. The CD spectrum for rVes v 5 with a minimum at 208 nm and a shoulder at 225 nm was superimposable to data reported for native Ves v 5.
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Additional file 3:
Serological data of patients assessed in basophil activation. sIgE levels for HBV (i1) and YJV (i3) were determined with the Immulite 2000 (Siemens Healthcare Diagnostics).
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Additional file 4:
Serological data of patients assessed in IgE reactivity analysis. The sIgE levels for HBV (i1) and YJV (i3) were determined with the Immulite 2000 (Siemens Healthcare Diagnostics) or ImmunoCap 250 (Phadia). In singular cases sIgE values were not determined, but patients were positive in skin prick testing. For some patients sIgE values are expressed by classes according to the manufacturer. The sIgE values to rVes v 1 and rVes v5 were considered as positive (+) at a OD 405 nm > 0.27. Cut off for high sIgE levels (++) was at OD 405 nm > 1.
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