|
Protein identification after 2DE-separation of proteins in sugar beet pollen extract |
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| Sample |
Rank |
Protein Name |
Acc Nr |
MW |
pI |
# |
|
|
||||||
| 1 |
1 |
CALM1_PETHY Calmodulin-1 (CaM-1) – Petunia hybrida |
P62199 |
16 763 |
4.1 |
6 |
| 2 |
1 |
CALM1_PETHY Calmodulin-1 (CaM-1) – Petunia hybrida |
P62199 |
16 763 |
4.1 |
5 |
| 3 |
1 |
PROF_CUCME Profilin (Pollen allergen Cuc m 2) – Cucumis melo (Muskmelon) |
Q5FX67 |
14 029 |
4.6 |
2 |
| 4 |
1 |
PROF_CUCME Profilin (Pollen allergen Cuc m 2) – Cucumis melo (Muskmelon) |
Q5FX67 |
14 029 |
4.6 |
2 |
| 5 |
1 |
CHE1_CHEAL Pollen allergen Che a 1 – Chenopodium album (Lamb's-quarters) |
Q8LGR0 |
18 739 |
5.0 |
2 |
| 2 |
SODC1_MESCR Superoxide dismutase – Mesembryanthemum crystallinum |
P93258 |
15 278 |
5.5 |
1 |
|
| 6 |
1 |
PMGI_MESCR 2,3-bisphosphoglycerate-independent phosphoglycerate mutase |
Q42908 |
61 316 |
5.4 |
1 |
| 2 |
SODC1_MESCR Superoxide dismutase – Mesembryanthemum crystallinum |
P93258 |
15 278 |
5.5 |
1 |
|
| 4 |
TRXH1_TOBAC Thioredoxin H-type 1 – Nicotiana tabacum |
P29449 |
14 118 |
5.6 |
1 |
|
| 5 |
CHE1_CHEAL Pollen allergen Che a 1 – Chenopodium album (Lamb's-quarters) |
Q8LGR0 |
18 739 |
5.0 |
3 |
|
| 7 |
1 |
DYH1A_CHLRE Dynein-1-alpha heavy chain – Chlamydomonas reinhardtii |
Q9SMH3 |
52 5420 |
5.3 |
1 |
| 8 |
1 |
CALM1_PETHY Calmodulin-1 (CaM-1) – Petunia hybrida |
P62199 |
16 763 |
4.1 |
2 |
|
Samples were excised from 2DE (see Fig. 4A) and analyzed by LC-MS/MS. Protein identification after LC-MS/MS was performed with the software GPS Explorer and an in-house Mascot search engine. Settings: Precursor Tol: 15 ppm, MS/MS Fragment Tol: 0.15 Da. For each protein identification, columns show: Accession number in the Swiss-Prot database (Acc Nr), Protein theoretical mass in Da (MW), Protein theoretical isoelectric point (pI), and the number of peptides used for protein identification (#). The Mascot Best ion score (i.e. the highest score of a single peptide), and the significance of the database search (C.I. = the confidence interval) calculated by the GPS Explorer software were > 45 with at a confidence interval (C.I.) > 99.0 for all peptides except for Che a 1 in sample 6 where Best ion score was 17 and C.I. = 90.1%. Best ion score was 56 and C.I. = 99.999% for the Che a 1-homologue in sample 5. Best ion core was 82 and 62 with C.I. = 100% for the Che a 2-homologue in sample 3 and 4. | ||||||
Luoto et al. Clinical and Molecular Allergy 2008 6:7 doi:10.1186/1476-7961-6-7 |
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