Electrophoresis gel lane of mesquite antigen (left) and mesqite allergens demonstrated by Western blotting (right). Pooled allergic human serum produced thirteen IgE responses to mesquite tree pollen (Prosopis juliflora var. glandulosa) when tested by Western blotting. The molecular weights of these allergens ranged from 11 to 99 kd when they were blotted from a 12% electrophoresis gel onto nitrocellulose as detected by the Amplified Opti-4-CN system (Bio-Rad).
Killian and McMichael Clinical and Molecular Allergy 2004 2:8 doi:10.1186/1476-7961-2-8