Effects of Sidestream cigarette smoke extract (Ss) on IL-8 production from IL-1β-activated HMC-1 cells. To each well of a 6-well culture plate, two ml of HMC-1 (1 × 106 cells/ml) were cultured alone (Medium), or in the presence of IL-1β (10 ng/ml), and the combinations of IL-1β with different concentrations of Ss for 24 hrs in triplicate. Supernatants were harvested for measuring IL-8 by ELISA.
Chi et al. Clinical and Molecular Allergy 2012 10:3 doi:10.1186/1476-7961-10-3