Figure 1.

Study design to generate acute allergic asthma phenotype in mice: Mice were sensitized with OVA (100 μg) complexed with aluminium sulfate in a 0.2-ml volume, administered by i.p. injection on day 0 and later challenged with OVA On days 8 (250 μg of OVA) and on days 15, 18, and 21 (125 μg of OVA), (Pierce, Rockford, IL) by intra-tracheal instillation. Mice were immunized with OVA (100 μg) complexed with aluminium sulfate in a 0.2-ml volume, administered by i.p. injection on day 0. The control group received normal saline with aluminium sulfate by i.p. route on day 0 and 0.05 ml of 0.9% saline by i.t. route on days 8, 15, 18, and 21. They were sacrificed on d22. The abbreviations used are: i.p. intra-peritoneal; i.t. intra-tracheal; BAL, bronchoalveolar lavage; PB, peripheral blood.